| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Kolessar, Kaitlyn R (7)
|
MADST |
42.21 |
40.53 |
-1.68 |
| 2 |
DeVose, Shelby Y (8)
|
MAJRAY |
41.93 |
42.36 |
0.43 |
| 3 |
Spittle, Olivia R (8)
|
MADST |
45.62 |
43.56 |
-2.06 |
| 4 |
Nelson, Addison E (8)
|
MADST |
48.55 |
47.09 |
-1.46 |
| 5 |
Enakhimion, Emiola A (8)
|
MAEAAC |
47.93 |
49.14 |
1.21 |
| 6 |
Bagi, Kira E (8)
|
MAACST |
|
49.37 |
- |
| 7 |
Hung, Danielle Hana T (8)
|
MAACST |
|
49.38 |
- |
| 8 |
Walder, Mickaeli A (8)
|
MADST |
52.16 |
49.45 |
-2.71 |
| 9 |
DeBaecke, Emerson G (7)
|
MADST |
52.97 |
49.61 |
-3.36 |
| 10 |
Kleinmeulman, Cayla E (8)
|
MADST |
46.98 |
49.71 |
2.73 |
| 11 |
Radziewicz, Gabriella E (7)
|
MADST |
47.66 |
50.05 |
2.39 |
| 12 |
O'Connor, Maeve O (8)
|
MASAKA |
52.33 |
50.23 |
-2.10 |
| 13 |
Garrabrant, Megan S (8)
|
MANRG |
50.80 |
50.44 |
-0.36 |
| 14 |
Fetter, Sarah E (8)
|
MAPRSC |
50.54 |
50.79 |
0.25 |
| 15 |
Wiley, Sadie K (8)
|
MANRG |
49.02 |
51.40 |
2.38 |
| 16 |
Muhammad, Safiyah M (7)
|
MASAKA |
48.50 |
51.47 |
2.97 |
| 17 |
Keller, Abigail L (8)
|
MADST |
53.38 |
51.54 |
-1.84 |
| 18 |
Ryan, Maxalyn E (6)
|
MADST |
52.00 |
52.83 |
0.83 |
| 19 |
Chen, Felicity Y (8)
|
MADST |
|
55.72 |
- |
| 20 |
Gelak, Lauren V (8)
|
MAJRAY |
53.74 |
56.02 |
2.28 |
| 21 |
Ambrose, Kate I (8)
|
MADST |
51.19 |
56.74 |
5.55 |
| 22 |
Schlosser, Ava G (7)
|
MADST |
|
56.75 |
- |
| 23 |
Ovalle, Mariana (8)
|
MAACST |
|
56.80 |
- |
| 24 |
Belair, Lilly J (6)
|
MADST |
|
58.87 |
- |
| 25 |
Ottes, Alyssa (8)
|
MANRG |
58.20 |
58.94 |
0.74 |
| 26 |
Ciaverelli, Cecelia G (8)
|
MANRG |
1:06.12 |
1:01.96 |
-4.16 |
| 27 |
Chapman, Leah H (5)
|
MADST |
1:04.62 |
1:05.06 |
0.44 |
| 28 |
O'Connor, Grian A (6)
|
MASAKA |
|
1:07.11 |
- |
| 29 |
Sbriglia, Isabella (6)
|
MADST |
|
1:17.82 |
- |
|
Alamo-Rosas, Raquel A (7)
|
MASAKA |
55.35 |
DQ |
- |
|
Klein, Amanda P (7)
|
MAJRAY |
1:11.30 |
NS |
- |
|
Williams, Victoria A (6)
|
MASAKA |
1:01.21 |
NS |
- |